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Antibiotic Production Through Solid-State Fermentation Under a Novel Fixed-Bed Micro-Reactor

In the present study, solid state fermentation (SSF) was evaluated using a novel PolyHIPE Polymer (PHP) matrix. This matrix was developed with an approach to improve the production of antibiotics. For the production of the matrix, a batch reactor was operated with a mixing speed of 300 rpm at differents mixing times (5, 10, 15 min) as a consequence, differents pores sizes was Obtained (55, 39, 19 μm) with a sulfonated/neutralized surface chemistry. After functionalization and purification, the matrix was placed in a fixed-bed micro-reactor, designed specifically for this project; which had 16 fixed beds for the production of antibiotics in the solid state using a model filamentous bacteria Streptomyces coelicolor A3(2). The growth conditions such as the size of the pores of the matrix, were investigated in relation to the growth time (From 0 to 168 hours), and two extracts were produced, Prodigiosin a member of the family of red pigment tripyrrol and Actinorhodin a benzoisochromoquinone dimeric antibiotic that belongs to a class of aromatic polykets. The concentration of the extracted antibiotics and their activities were examined by the disc diffusion method. The two compounds produced were tested against microbial strains (Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas fluorescens, and Penicillium notatum) and the inhibition effects were measured. The results concluded that the highest specific production rate of prodigiosin (3.02 μmol ml-1h-1) and actinorhodin (26.08 μmol ml-1h-1), was achieved within the PHP matrix, with pore sizes of 39 μm and 19 μm in diameter respectively. On the other hand, the assay revealed a larger inhibition halo (diameter in mm) that was observed in the plate inoculated with DSM 10 strains (Bacillus subtilis), inhibited by Prodigiosin extract.

PolyHIPE Polymer (PHP), Solid State Fermentation (SSF), Streptomyces coelicolor A3(2) Prodigiosin, Actinorhodin, Antimicrobial Screening

Teresa Matoso Manguangua Victor, Jarka Glassey, Kristie Kamps, Alan Claude Ward. (2023). Antibiotic Production Through Solid-State Fermentation Under a Novel Fixed-Bed Micro-Reactor. American Journal of Chemical Engineering, 11(4), 64-74.

Copyright © 2023 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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